Development of a Multiplex PCR Assay for the Detection of Metallo-Beta-Lactamase Genes in Pseudomonas aeruginosa

The appearance of Pseudomonas aeruginosa strains with multi-resistance to antibiotics is a clinical problem great relevance. methods for detecting these resistances are laborious and slow, which complication when treating patients promptly. In this work, we developed simple method simultaneous detection several carbapenem resistance genes using multiplex PCR assay. assay developed, followed by electrophoretic separation fragments, allows simultaneously identify the presence 6 antibiotic genes: bla-VIM (261 bp), bla-IMP (587 bla-SPM (648 bla-GIM-1 (753 bla-NDM-1 (813 bp) bla-KPC (882 bp). We analyzed 7 isolates P. obtained in Chile, finding bla-VIM, bla-IMP, bla-SPM, bla-GIM, bla-NDM 5 them. found perfect correlation between various results antibiograms. Interestingly, 2 possessed 3 different simultaneously. Finally, never described before Chile (bla-IMP, bla-GIM-1). rapid test up metallo-β-lactamase family aeruginosa.